5Y9V

Crystal structure of diamondback moth ryanodine receptor N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	170.130, 170.130, 51.763
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.864 - 2.841
R-factor	0.2163
Rwork	0.213
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ila
RMSD bond length	0.004
RMSD bond angle	0.702
Data scaling software	HKL-2000
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.240	2.940
High resolution limit [Å]	2.841	2.840
Number of reflections	20497
<I/σ(I)>	18.9
Completeness [%]	99.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298.15	0.1 M HEPES pH 7.0, 1.6 M ammonium sulfate