5Y9S
Crystal structure of VV2_1132, a LysR family transcriptional regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801, 1.0082 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 113.669, 221.895, 57.875 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.769 - 2.199 |
R-factor | 0.2048 |
Rwork | 0.202 |
R-free | 0.26360 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.955 |
Data scaling software | HKL-2000 (v712) |
Phasing software | PHENIX ((1.11.1_2575)) |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.199 | 2.200 |
Rmerge | 0.061 | 2.500 |
Rpim | 0.019 | 0.214 |
Number of reflections | 70737 | |
<I/σ(I)> | 50.21 | |
Completeness [%] | 93.7 | 83.8 |
Redundancy | 8.3 | 4.6 |
CC(1/2) | 0.219 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 287 | 0.1M imidazol (pH 7.6), 0.9M ammonium phosphate dibasic, 0.2M NaBr, 2mM TCEP |