5Y9P

Staphylococcus aureus RNase HII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	61.830, 61.830, 163.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.23792
Rwork	0.236
R-free	0.28231
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2etj
RMSD bond length	0.016
RMSD bond angle	1.365
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.820
High resolution limit [Å]	2.200
Number of reflections	16871
<I/σ(I)>	18.7
Completeness [%]	99.8
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.15M ammonium sulfate, 0.1M MES, 15%(w/v) PEG 4000