5Y9N

Crystal structure of Pyrococcus furiosus PbaA (monoclinic form), an archaeal homolog of proteasome-assembly chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-12-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	92.690, 200.940, 92.760
Unit cell angles	90.00, 110.96, 90.00

Refinement procedure

Resolution	20.000 - 2.550
R-factor	0.20939
Rwork	0.208
R-free	0.24039
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wz2
RMSD bond length	0.012
RMSD bond angle	1.549
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.550	2.550
Rmerge	0.043	0.675
Number of reflections	101818	16291
<I/σ(I)>	17.5	1.9
Completeness [%]	99.0	98.3
Redundancy	3.8	3.8
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	30% MPD, 0.1 M sodium acetate (pH 4.6), and 20 mM calcium chloride