5Y9F
Crystal structure of HPV59 pentamer in complex with the Fab fragment of antibody 28F10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-09-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.265, 417.233, 134.942 |
| Unit cell angles | 90.00, 111.02, 90.00 |
Refinement procedure
| Resolution | 36.528 - 3.350 |
| R-factor | 0.2316 |
| Rwork | 0.230 |
| R-free | 0.26120 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.649 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.350 |
| Number of reflections | 161106 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 94.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2% tacsimate, 0.1 M sodium citrate, pH 5.5, 17% (w/v) PEG 3350 |
