5Y9C

Crystal structure of HPV58 pentamer in complex with the Fab fragment of antibody A12A3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2012-09-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	121.571, 102.600, 138.032
Unit cell angles	90.00, 114.52, 90.00

Refinement procedure

Resolution	47.861 - 3.443
R-factor	0.2046
Rwork	0.202
R-free	0.24920
RMSD bond length	0.002
RMSD bond angle	0.507
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.450
Number of reflections	41030
<I/σ(I)>	10.4
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M lithium chloride, 0.1 M Tris pH 8.0, 14% (w/v) PEG 3350