5Y9C
Crystal structure of HPV58 pentamer in complex with the Fab fragment of antibody A12A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2012-09-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 121.571, 102.600, 138.032 |
Unit cell angles | 90.00, 114.52, 90.00 |
Refinement procedure
Resolution | 47.861 - 3.443 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.24920 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.507 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.450 |
Number of reflections | 41030 |
<I/σ(I)> | 10.4 |
Completeness [%] | 99.9 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M lithium chloride, 0.1 M Tris pH 8.0, 14% (w/v) PEG 3350 |