5Y9B
Crystal structure of Bacillus licheniformis Gamma glutamyl transpeptidase with DON
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-03-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.560, 61.396, 145.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.150 |
| R-factor | 0.18026 |
| Rwork | 0.177 |
| R-free | 0.23609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xlu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.662 |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rpim | 0.024 | |
| Number of reflections | 29483 | 1288 |
| <I/σ(I)> | 29.39 | 2.73 |
| Completeness [%] | 97.1 | 86.4 |
| Redundancy | 10 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 4000, 100 mM NaCl, 200 mM MgCl2, MPD, 100 mM Tris-Cl |
