5Y97
Crystal structure of snake gourd seed lectin in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 109.429, 109.429, 232.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.770 - 3.050 |
| R-factor | 0.22287 |
| Rwork | 0.222 |
| R-free | 0.25067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hr6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.959 |
| Data reduction software | iMOSFLM (7.2) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.7.15) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.770 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.146 | 1.534 |
| Number of reflections | 16493 | 2340 |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.9 | 19.5 |
| CC(1/2) | 0.990 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 300 | reservoir solution contained 4M sodium formate |
