5Y91
The structure of the MHC class I molecule of bony fishes provides insights into the conserved nature of the antigen-presenting system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.280, 50.413, 86.077 |
| Unit cell angles | 90.00, 124.08, 90.00 |
Refinement procedure
| Resolution | 32.883 - 1.901 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yez |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.765 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 31840 |
| <I/σ(I)> | 17.711 |
| Completeness [%] | 99.0 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M ammonium citrate tribasic, pH 7.0 and 20% w/v polyethylene glycol 3,350 |
