5Y8V
Crystal structure of GAS41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.467, 57.212, 205.854 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.495 - 2.610 |
R-factor | 0.239 |
Rwork | 0.237 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fk3 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.501 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 19176 |
<I/σ(I)> | 9.51 |
Completeness [%] | 98.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 291 | 0.1M MES pH 6.5, 1.6M MgSO4 |