5Y8U
Crystal structure of the C276S mutant of MAP2K7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.534, 71.534, 262.305 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.950 - 2.920 |
| R-factor | 0.23362 |
| Rwork | 0.230 |
| R-free | 0.29880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b2k |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.950 | 2.970 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.095 | 0.802 |
| Number of reflections | 9446 | |
| <I/σ(I)> | 34.7 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG3350, sodium citrate |
