5Y8U
Crystal structure of the C276S mutant of MAP2K7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 71.534, 71.534, 262.305 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.950 - 2.920 |
R-factor | 0.23362 |
Rwork | 0.230 |
R-free | 0.29880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b2k |
RMSD bond length | 0.012 |
RMSD bond angle | 1.689 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.950 | 2.970 |
High resolution limit [Å] | 2.920 | 2.920 |
Rmerge | 0.095 | 0.802 |
Number of reflections | 9446 | |
<I/σ(I)> | 34.7 | 4.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 | 21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG3350, sodium citrate |