5Y87
Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.65 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.221, 108.963, 41.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.073 - 2.132 |
| R-factor | 0.2051 |
| Rwork | 0.202 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.130 |
| Number of reflections | 44790 |
| <I/σ(I)> | 23.4 |
| Completeness [%] | 100.0 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M NaOAc, pH 5.2, 0.15M CaCl2, 18-22% MPD, 50-100mM MnCl2 |
