5Y85
Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 91.182, 109.304, 41.739 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 35.852 - 2.001 |
| R因子 | 0.2189 |
| Rwork | 0.218 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 50.000 |
| 分解能 [Å] (高) | 2.000 |
| 独立反射数 | 28779 |
| <I/σ(I)> | 16.4 |
| 完全性 [%] | 99.7 |
| 冗長性 | 4.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M NaOAc, pH 5.2, 0.15M CaCl2, 18-22% iso-propanol or MPD |
