5Y83

Crystal structure of YidC from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	75.745, 103.600, 129.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.758 - 3.842
R-factor	0.266
Rwork	0.264
R-free	0.31240
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-2000
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.940
High resolution limit [Å]	3.800	8.180	3.800
Rmerge	0.120	0.061
Rmeas	0.128	0.066
Rpim	0.054	0.024	0.940
Total number of observations	85442
Number of reflections	10459	1118	1019
<I/σ(I)>	4.4
Completeness [%]	99.7	97.1	100
Redundancy	8.2	7.5	8.3
CC(1/2)		0.997	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	289	0.1M HEPES (pH 7.5), 20% PEG1500(w/v)