5Y82

Crystal structure of the periplasmic domain of the Thermotoga maritima YidC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 41
Unit cell lengths	79.952, 79.952, 135.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.966 - 2.517
R-factor	0.2226
Rwork	0.221
R-free	0.26080
Structure solution method	SAD
Data scaling software	HKL-2000 (v706)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.540
High resolution limit [Å]	2.500	6.760	2.500
Rmerge	0.129	0.102
Rmeas	0.134	0.108
Rpim	0.045	0.034	0.484
Total number of observations	293966
Number of reflections	28926	1478	1443
<I/σ(I)>	8.4
Completeness [%]	99.9	99.5	99.9
Redundancy	10.2	10	9.6
CC(1/2)		0.980	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9	289	0.05M Glycine (pH 9.0) and 55%PEG400(w/v)