5Y7Q

Crystal structure of paFAN1 bound to 2nt 5'flap DNA with gap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	80.845, 104.560, 105.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 2.700
R-factor	0.1959
Rwork	0.193
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r8a
RMSD bond length	0.009
RMSD bond angle	1.079
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1-2155_1069: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.490
Rmerge	0.092	2.416
Rpim		0.649
Number of reflections	31458
<I/σ(I)>	35.5
Completeness [%]	99.0
Redundancy	14.4
CC(1/2)		0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	0.1M HEPES (pH7.2), 25%(w/v) PEG 8000, 30mM Gly-gly-gly