5Y7P
Bile salt hydrolase from lactobacillus salivarius complex with glycocholic acid and cholic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-27 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.010, 94.090, 166.974 |
Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
Resolution | 28.500 - 2.100 |
R-factor | 0.16268 |
Rwork | 0.160 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hke |
RMSD bond length | 0.016 |
RMSD bond angle | 1.682 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.073 | |
Number of reflections | 143414 | 6716 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 95.1 | 89.7 |
Redundancy | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 20% polyethylene glycol 3350, 0.2 M potassium dihydrogen phosphate pH 4.8. Then crystal is soaked in this buffer containing 5 uM glycocholic acid for 4 hrs. |