5Y7P
Bile salt hydrolase from lactobacillus salivarius complex with glycocholic acid and cholic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-27 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.010, 94.090, 166.974 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 28.500 - 2.100 |
| R-factor | 0.16268 |
| Rwork | 0.160 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hke |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.682 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.073 | |
| Number of reflections | 143414 | 6716 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 95.1 | 89.7 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 20% polyethylene glycol 3350, 0.2 M potassium dihydrogen phosphate pH 4.8. Then crystal is soaked in this buffer containing 5 uM glycocholic acid for 4 hrs. |
