5Y7O
Crystal structure of folding sensor region of UGGT from Thermomyces dupontii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-04-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.97864 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 195.090, 195.090, 142.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.970 - 3.100 |
R-factor | 0.2352 |
Rwork | 0.232 |
R-free | 0.27830 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 2.269 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.290 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.042 | 0.639 |
Number of reflections | 105778 | 17378 |
<I/σ(I)> | 14.4 | 1.4 |
Completeness [%] | 96.4 | 98.3 |
Redundancy | 2.7 | 2.8 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.95 M Ammonium sulfate, 0.1 M Tris-HCl (pH 7.0), 8 mM n-decanoylsucrose |