5Y7I
Structure of tilapia fish CLIC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.230, 44.730, 71.470 |
| Unit cell angles | 90.00, 97.91, 90.00 |
Refinement procedure
| Resolution | 37.745 - 3.000 |
| R-factor | 0.2287 |
| Rwork | 0.227 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r5g |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.714 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.830 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.066 | 0.575 |
| Number of reflections | 9822 | |
| <I/σ(I)> | 15.8 | 3.3 |
| Completeness [%] | 94.1 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 24% (w/v) PEG 2000 MME, 100mM Tris base/ Hydrochloric acid pH 8.5, 220mM Trimethylamine n-oxide |
