5Y7F

Crystal structure of catalytic domain of UGGT (UDP-bound form) from Thermomyces dupontii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2016-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	45.410, 46.850, 131.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.350
R-factor	0.17054
Rwork	0.169
R-free	0.20759
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h18
RMSD bond length	0.014
RMSD bond angle	1.671
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.350	1.350
Rmerge	0.088	0.796
Number of reflections	62275	9467
<I/σ(I)>	12.8
Completeness [%]	99.1	94.5
Redundancy	7	6.2
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	Crystallization buffer 24% PEG 3350, 100 MM TRIS-HCL (PH 9.0), Soaking buffer 24% PEG 3350, 100 MM TRIS-HCL (PH 9.0), 2 mM CaCl2, 10 mM UDP