5Y79

Crystal structure of the triose-phosphate/phosphate translocator in complex with 3-phosphoglycerate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	107.050, 165.330, 41.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.998 - 2.200
R-factor	0.1926
Rwork	0.191
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i20
RMSD bond length	0.003
RMSD bond angle	0.637
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	38123
<I/σ(I)>	13.3	1.3
Completeness [%]	100.0	99.9
Redundancy	39.2	27
CC(1/2)	0.994	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	PEG200, Na-citrate, 3K-citrate, 2Na(3-PGA)