5Y72
DMSPP Bound AmbP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.394, 55.080, 77.668 |
| Unit cell angles | 105.41, 106.02, 98.79 |
Refinement procedure
| Resolution | 43.953 - 1.650 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y4g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.506 | |
| Number of reflections | 79907 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 96.1 | 94.3 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MES, PEG 8000, magnesium chloride |
