5Y6Z

Crystal structure of the coxsackievirus A16 polymerase elongation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 31
Unit cell lengths	93.724, 93.724, 167.512
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.829 - 2.500
R-factor	0.2202
Rwork	0.218
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ol6
RMSD bond length	0.008
RMSD bond angle	0.985
Data reduction software	d*TREK (9.9.9.4D)
Data scaling software	d*TREK (9.9.9.4D)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.030	46.030	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.132	0.088	0.372
Rmeas	0.158	0.107	0.448
Number of reflections	56671	5623	5705
<I/σ(I)>	4.7	8.6	2.1
Completeness [%]	99.5	98.6	99.9
Redundancy	2.93	2.88	2.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	ammonium dihydrogen phosphate