5Y6T

Crystal structure of endo-1,4-beta-mannanase from Eisenia fetida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	50.130, 69.461, 86.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.200 - 1.700
R-factor	0.13238
Rwork	0.130
R-free	0.16927
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c0h
RMSD bond length	0.018
RMSD bond angle	1.903
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	1.810
High resolution limit [Å]	1.700	1.700
Rmeas	0.088	0.554
Number of reflections	33115	10039
<I/σ(I)>	13.1	2.7
Completeness [%]	98.4	97.1
Redundancy	3.4	3.3
CC(1/2)	0.998	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1 M ammonium citrate, 15%(v/v) iso-propanol, 0.1 M Tris-HCl pH 8.5