5Y6I

Crystal structure of Pseudomonas aeruginosa HmgR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-01-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 2 21
Unit cell lengths	60.129, 80.520, 116.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.178 - 2.517
R-factor	0.2398
Rwork	0.237
R-free	0.29540
RMSD bond length	0.009
RMSD bond angle	1.134
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	19620
<I/σ(I)>	27.2
Completeness [%]	99.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M sodium citrate tribasic dehydrate, 20% w/v polyethylene glycol 3350