5Y6G

PilZ domain with c-di-GMP of YcgR from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	110.340, 110.340, 35.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.779 - 2.300
R-factor	0.2048
Rwork	0.204
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y6f
RMSD bond length	0.002
RMSD bond angle	0.558
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.780	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.094	0.612
Rpim	0.031	0.203
Number of reflections	11266	1624
<I/σ(I)>	16	4.1
Completeness [%]	99.9	100
Redundancy	10.1	9.9
CC(1/2)	0.998	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5, 23% PEG 3350