5Y63
Crystal structure of Enterococcus faecalis AhpC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.191, 124.674, 97.788 |
| Unit cell angles | 90.00, 113.66, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.870 |
| R-factor | 0.21001 |
| Rwork | 0.208 |
| R-free | 0.25171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.786 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 25934 | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 99.1 | 95.4 |
| Redundancy | 3.2 | 2.2 |
| CC(1/2) | 0.992 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Tris HCl pH 8.5, Magnesium Chloride, PEG 400 (30%) |
