5Y62
YfiR complexed with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 120.297, 120.297, 88.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.727 - 3.000 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yn7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8.2_1309) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.730 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 13528 | 667 |
| <I/σ(I)> | 22.12 | 5.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 25.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes (pH 7.5), 2 M ammonium sulfate |
