5Y5A

Crystal structure of Est1 and Cdc13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97860
Spacegroup name	C 1 2 1
Unit cell lengths	108.611, 41.726, 147.060
Unit cell angles	90.00, 97.45, 90.00

Refinement procedure

Resolution	40.856 - 2.206
R-factor	0.178
Rwork	0.176
R-free	0.22450
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.852
Data scaling software	HKL-3000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.101	0.070	0.594
Rmeas	0.110	0.076	0.652
Rpim	0.042	0.029	0.262
Total number of observations	194302
Number of reflections	30712	3341	3087
<I/σ(I)>	5.5
Completeness [%]	91.8	95.5	93.6
Redundancy	6.3	6.6	5.8
CC(1/2)		0.996	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	0.2 M potassium chloride, and 20% PEG3350
1	EVAPORATION	7	277	0.2 M potassium chloride, and 20% PEG3350