5Y53
Crystal structure of AL2 PAL domain in complex with AtBMI1b binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.650, 49.232, 71.265 |
| Unit cell angles | 75.64, 82.97, 67.38 |
Refinement procedure
| Resolution | 28.603 - 1.598 |
| R-factor | 0.1557 |
| Rwork | 0.155 |
| R-free | 0.18910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xvl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-3000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.598 | 1.600 |
| Rmerge | 0.320 | |
| Number of reflections | 69212 | 6945 |
| <I/σ(I)> | 15.9 | 3.7 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 7.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% PEG 2000 MME, 0.1 M HEPES, pH 7.5 |
