5Y53
Crystal structure of AL2 PAL domain in complex with AtBMI1b binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 |
Unit cell lengths | 43.650, 49.232, 71.265 |
Unit cell angles | 75.64, 82.97, 67.38 |
Refinement procedure
Resolution | 28.603 - 1.598 |
R-factor | 0.1557 |
Rwork | 0.155 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xvl |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | HKL-3000 |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.660 |
High resolution limit [Å] | 1.598 | 1.600 |
Rmerge | 0.320 | |
Number of reflections | 69212 | 6945 |
<I/σ(I)> | 15.9 | 3.7 |
Completeness [%] | 98.6 | 98.5 |
Redundancy | 7.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% PEG 2000 MME, 0.1 M HEPES, pH 7.5 |