5Y50

Crystal structure of eukaryotic MATE transporter AtDTX14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-09
Detector	RAYONIX MX225-HS
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	52.800, 86.780, 116.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.345 - 2.600
R-factor	0.2266
Rwork	0.224
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mkt
RMSD bond length	0.008
RMSD bond angle	0.943
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.345	48.345	2.760
High resolution limit [Å]	2.600	7.780	2.600
Rmerge	0.576	0.130	6.712
Rmeas	0.587	0.133	6.833
Number of reflections	16880	728	2767
<I/σ(I)>	7.79	28.45	0.9
Completeness [%]	98.5	99.2	100
Redundancy	28.488	25.054	28.482
CC(1/2)	0.994	0.997	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	Na-citrate, MgSO4, NaK-tartrate-tetrahydrate, PEG550MME