5Y4U
Crystal structure of Grx domain of Grx3 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.195, 45.749, 45.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.460 - 1.700 |
| R-factor | 0.18234 |
| Rwork | 0.180 |
| R-free | 0.22948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zyw |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.844 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 10493 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 97.0 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1 M Tris-HCl, pH 8.0, 2 M Ammonium sulfate |
