5Y4T
Crystal structure of Trx domain of Grx3 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.329, 77.329, 37.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.580 - 1.400 |
| R-factor | 0.19004 |
| Rwork | 0.189 |
| R-free | 0.21907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d6i |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.403 |
| Rmerge | 0.046 | |
| Number of reflections | 22509 | |
| <I/σ(I)> | 40 | 2.8 |
| Completeness [%] | 99.6 | 96.44 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289.15 | 0.1 M Tris-HCl, pH 8.0, 2 M Ammonium sulfate |
