5Y4S

Structure of a methyltransferase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.97795
Spacegroup name	I 41
Unit cell lengths	279.545, 279.545, 138.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.417 - 3.405
R-factor	0.2626
Rwork	0.260
R-free	0.29280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y4r
RMSD bond length	0.004
RMSD bond angle	1.093
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.610
High resolution limit [Å]	3.405	3.410
Number of reflections	143861
<I/σ(I)>	13.82	1.42
Completeness [%]	99.9	99.8
Redundancy	14.5	13.68
CC(1/2)	0.998	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium phosphate, potassium phosphate