5Y4C
Crystal structure of EfeO-like protein Algp7 in complex with a metal ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.626, 56.638, 71.173 |
| Unit cell angles | 90.00, 122.61, 90.00 |
Refinement procedure
| Resolution | 30.551 - 2.701 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3at7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.430 |
| Number of reflections | 7437 | |
| <I/σ(I)> | 21.9 | 3.33 |
| Completeness [%] | 98.8 | 93.5 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% polyethylene glycol 1500, 0.1M sodium 4-(2-hydroxyethyl)-1-piperazineethanesulfonate |
