5Y45
Crystal structure of a collagen-like peptide with interruption sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 13.750, 45.440, 77.630 |
| Unit cell angles | 90.00, 90.67, 90.00 |
Refinement procedure
| Resolution | 45.440 - 1.030 |
| R-factor | 0.1457 |
| Rwork | 0.144 |
| R-free | 0.17540 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.553 |
| Data scaling software | Aimless |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.440 |
| High resolution limit [Å] | 1.030 |
| Number of reflections | 42193 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 94.4 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M HEPES pH 7.0, 30% v/v Jeffamine, M-600 |
