5Y3Z
Structure of the periplasmic domain of the MotB L119P mutant from Salmonella (crystal form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.130, 84.800, 86.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.687 - 2.000 |
R-factor | 0.1927 |
Rwork | 0.190 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zvy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.714 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.400 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.093 | 0.496 |
Number of reflections | 22684 | 1629 |
<I/σ(I)> | 10.7 | 2.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 28% PEG 3350, 0.1M imidazole-HCl, 0.15M Arg-HCl, 5% MPD |