5Y3C

Crystal structure of zebrafish Ccd1 DIX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-21
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 61
Unit cell lengths	46.724, 46.724, 79.361
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.362 - 1.960
R-factor	0.1739
Rwork	0.169
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y3b
RMSD bond length	0.004
RMSD bond angle	0.757
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.960
Number of reflections	7076
<I/σ(I)>	35.3
Completeness [%]	99.1
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	283	14% poly(acrylic acid sodium salt) 5100, 100 mM HEPES-Na (pH 7.5), 20 mM MgCl2, 10 mM TCEP-HCl