5Y2M
Crystal structure of a group 2 HA binding antibody AF4H1K1 Fab in complex with the H4N6 duck isolate (H4-CZ/56) hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.175, 140.940, 138.100 |
| Unit cell angles | 90.00, 95.31, 90.00 |
Refinement procedure
| Resolution | 49.212 - 3.800 |
| R-factor | 0.2377 |
| Rwork | 0.235 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xl1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 43204 | 4301 |
| <I/σ(I)> | 0.255 | 1.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M Bis-Tris pH6.5, 10% polyethylene glycol 10,000 |
