5Y2L
Crystal structure of a group 2 HA binding antibody AF4H1K1 Fab in complex with the 1968 H3N2 pandemic (H3-AC/68) hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 157.482, 157.482, 355.344 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.331 - 2.902 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.24360 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.164 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.097 | |
Number of reflections | 37831 | 3739 |
<I/σ(I)> | 23 | 2.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.7 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.19 mM CYMAL7, 0.1M HEPES pH 7.5, 40% ployethylene glycol 400 |