5Y2G
Structure of MBP tagged GBS CAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2012-03-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 145.620, 145.620, 156.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.184 - 3.000 |
| R-factor | 0.2459 |
| Rwork | 0.238 |
| R-free | 0.31530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ifp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.221 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20079 | |
| <I/σ(I)> | 32 | 1.9 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 20.9 | 21.4 |
| CC(1/2) | 1.000 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.5 M Ammonium Sulfate, 0.1 M HEPES, pH 7.0 |
