5Y2E

Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain NCDV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.350, 48.140, 122.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.380 - 2.700
R-factor	0.2389
Rwork	0.237
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.728
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	61.380
High resolution limit [Å]	2.700
Number of reflections	7012
<I/σ(I)>	8.8
Completeness [%]	94.2
Redundancy	4
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.6	289	0.1M tri-Sodium citrate dehydrate, 0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate