5Y24

Crystal structure of AimR from Bacillus phage SPbeta in complex with its signalling peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.92014
Spacegroup name	P 2 21 21
Unit cell lengths	33.550, 119.605, 214.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.196 - 1.922
R-factor	0.2179
Rwork	0.216
R-free	0.24890
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.976
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.920
Number of reflections	67332
<I/σ(I)>	39.9
Completeness [%]	99.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 8000, dithiothreitol, Sodium Bromine, Sodium Cacodylate