5Y21
Crystal structure of AL2 PAL domain in complex with AtRing1a proximal site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.114, 70.563, 55.324 |
| Unit cell angles | 90.00, 112.78, 90.00 |
Refinement procedure
| Resolution | 27.791 - 1.769 |
| R-factor | 0.1638 |
| Rwork | 0.162 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xvl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.746 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.769 | 1.770 |
| Rmerge | 0.154 | |
| Number of reflections | 31734 | 3179 |
| <I/σ(I)> | 35.4 | 14.4 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% PEG2000 MME, 0.1 M Tris, pH 7.5 |
