5Y1F
Monomeric L-threonine 3-dehydrogenase from metagenome database (NAD+ bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.494, 48.927, 56.364 |
| Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
| Resolution | 54.890 - 1.250 |
| R-factor | 0.15827 |
| Rwork | 0.157 |
| R-free | 0.18055 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.652 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 75876 | |
| <I/σ(I)> | 28.9 | |
| Completeness [%] | 91.5 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 3350, 0.2M Sodium fluoride |
