5Y11

SFTSV GN with neutralizing antibody MAb4-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97905
Spacegroup name	P 21 21 21
Unit cell lengths	82.605, 103.194, 112.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.753 - 2.100
R-factor	0.1709
Rwork	0.169
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.088	0.281
Number of reflections	56517	5327
<I/σ(I)>	22.7
Completeness [%]	99.5	95.8
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.3M potassium/sodium tartrate, 20% (w/v) PEG 3350, 0.1M Bis Tris propane pH 7.5