5Y10
SFTSV Gn head domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97853 |
| Spacegroup name | I 4 |
| Unit cell lengths | 87.410, 87.410, 91.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.643 - 2.600 |
| R-factor | 0.2237 |
| Rwork | 0.221 |
| R-free | 0.28510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.848 | |
| Number of reflections | 10777 | 1108 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 96.3 | 100 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 4000, 20% (v/v) 2-propanol, 0.1M sodium citrate pH 5.5 |
