5Y0W
The structure of RVFV Gn head domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.571, 73.874, 98.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.870 - 2.500 |
R-factor | 0.1994 |
Rwork | 0.197 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.787 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.104 | 0.774 |
Number of reflections | 13526 | 1286 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 99.5 | 96.5 |
Redundancy | 6.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M ammonium sulfate, 0.1M MES, pH 6.5, 20% (w/v) PEG 8000 |