5Y0O
Crystal structure of apo BsTmcAL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2014-12-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.755, 67.656, 210.805 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.774 - 2.300 |
R-factor | 0.1802 |
Rwork | 0.178 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y0n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.233 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.046 | 0.019 | 1.557 |
Rpim | 0.013 | 0.006 | 0.441 |
Number of reflections | 43997 | 2390 | 2205 |
<I/σ(I)> | 32.57 | 108.7 | 1.8 |
Completeness [%] | 100.0 | 99.87 | 99.91 |
Redundancy | 13.24 | 11.73 | 13.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-HCl, PEG400, KCl, MgCl2 |