5Y0L
Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72., D122G/H130Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.880, 144.003, 129.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.000 - 1.385 |
| R-factor | 0.17269 |
| Rwork | 0.171 |
| R-free | 0.19952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xyg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.688 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.410 |
| High resolution limit [Å] | 1.385 | 1.390 |
| Rmerge | 0.047 | 0.886 |
| Number of reflections | 128858 | 6285 |
| <I/σ(I)> | 38.5 | |
| Completeness [%] | 99.1 | 88.6 |
| Redundancy | 7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2-2.2 M ammonium sulphate, 0.2 M NaCl, 0.1 M HEPES buffer pH 7.5-8.5 |
